Area
| Access restrictions
| Bay Keys | No-motor zone (300 feet) around one key; idle speed only/no-wake zones in tidal creeks.
| Boca Grande Key | South one-half of beach closed (beach above mean high water closed by Department of the Interior).
| Woman Key | One-half of beach and sand spit on southeast side closed (beach and sand spit above mean high water closed by Department of the Interior).
| Cayo Agua Keys | Idle speed only/no-wake zones in all navigable tidal creeks.
| Cotton Key | No-motor zone on tidal flat.
| Snake Creek | No-motor zone on tidal flat.
| Cottrell Key | No-motor zone (300 feet) around entire key.
| Little Mullet Key | No-access buffer zone (300 feet) around entire key.
| Big Mullet Key | No-motor zone (300 feet) around entire key.
| Crocodile Lake | No-access buffer zone (100 feet) along shoreline between March 1 and October 1.
| East Harbor Key | No-access buffer zone (300 feet) around northernmost island.
| Lower Harbor Keys | Idle speed only/no-wake zones in selected tidal creeks.
| Eastern Lake Surprise | Idle speed only/no-wake zone east of highway U.S. 1.
| Horseshoe Key | No-access buffer zone (300 feet) around main island (main island closed by Department of the Interior).
| Marquesas Keys | (i) No-motor zones (300 feet) around three smallest keys on western side of chain; (ii) no-access buffer zone (300 feet) around one island at western side of chain; (iii) idle speed only/no-wake zone in southwest tidal creek.
| Tidal flat south of Marvin Key | No-access buffer zone on tidal flat.
| Mud Keys | (i) Idle speed only/no-wake zones in the two main tidal creeks; (ii) two smaller creeks on west side closed.
| Pelican Shoal | No-access buffer zone out to 50 meters from shore between April 1 and August 31 (shoal closed by the Florida Game and Freshwater Fish Commission).
| Rodriguez Key | No-motor zone on tidal flats.
| Dove Key | No-motor zone on tidal flats; area around the two small islands closed.
| Tavernier Key | No-motor zone on tidal flats.
| Sawyer Keys | Tidal creeks on south side closed.
| Snipe Keys | (i) Idle speed only/no-wake zone in main tidal creek; (ii) no-motor zone in all other tidal creeks.
| Upper Harbor Key | No-access buffer zone (300 feet) around entire key.
| East Content Keys | Idle speed only/no-wake zones in tidal creeks between southwesternmost keys.
| West Content Keys | Idle speed only/no-wake zones in selected tidal creeks; no-access buffer zone in one cove.
| Little Crane Key | No-access buffer zone (300 feet) around entire key. |